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IHC staining of purified anti-β-Amyloid, 1-16 antibody (clone 6E10) on the formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1 µg/mL of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm -
Western blot of purified anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blot was incubated with 0.025 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of the purified anti-β-Amyloid, 1-42 antibody (12F4) on the formalin-fixed paraffin-embedded brain tissues from normal human (left panel) and patients with Alzheimer’s disease (left panel) and normal human brain tissue (right panel). Following antigen retrieval using 70% formic acid for 20 min, the tissue was incubated overnight with 1µg/mL of the primary antibody at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Western blot of the purified anti-β-amyloid, 1-42 antibody (clone 12F4). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (cat: 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-β-Amyloid, 1-15 antibody (clone 3A1) on frozen normal (left panel) and Alzheimer's disease (right panel) human brain tissues. The tissues were incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
The Aβ Antibody Sampler Kit offers flexibility for sampling and detection of full-length amyloid precursor protein (APP) and various APP cleavage fragments including Aβ 1-40 and Aβ 1-42 peptides. The kit also includes clone 3A1, which offers unique advantages over gold standard antibodies for Aβ detection such as clones 6E10 and 4G8. In addition to detecting C-terminally processed fragments of APP, clone 3A1 shows preference for aggregated Aβ and demonstrates no cross-reactivity with APP. Clone M3.2 recognizes rodent Aβ, β-CTF, sAPP-α, and full-length APP. Clone M3.2 has negligible cross-reactivity with human isoforms of APP and Aβ. The antibodies provided in this kit are highly suitable for WB, IHC, and ELISA applications.
Product DetailsKit Contents
- Kit Contents
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Specificity Format Clone Size Reactivity Isotype Anti-β-Amyloid, 1-16 Purified 6E10 25 μl Human Mouse IgG1 Anti-mouse/rat β-Amyloid, 1-16 Purified M3.2 25 μl Mouse, Rat Mouse IgG1, κ Anti-β-Amyloid, 1-40 Purified 11A50-B10 50 μl Human Mouse IgG1 Anti-β-Amyloid, 1-42 Purified 12F4 50 μl Human, Mouse, Rat Mouse IgG1 Anti-β-Amyloid, 1-15 Purified 3A1 25 μg Human Mouse IgG1, κ * For detailed information about each specificity, please refer to the datasheets of the individual products.
Product Details
- Formulation
- Please refer to individual product datasheets of the purified formats for details.
- Preparation
- All antibodies in this kit were purified by affinity chromatography.
- Storage & Handling
- Upon receipt, store undiluted at 2-8°C.
- Application
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WB, IHC-P, IHC-F - Quality tested
- Recommended Usage
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Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:
Anti-β-Amyloid, 1-16: 0.2 - 1.0 µg/ml
Anti-mouse/rat β-Amyloid, 1-16: 0.1 - 5.0 µg/ml
For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested use of this reagent is as follows:
Anti-β-Amyloid, 1-40: 5.0 - 10 µg/ml
Anti-β-Amyloid, 1-42: 0.2 - 5.0 µg/ml
For immunohistochemistry on frozen tissue, the suggested use of this reagent is as follows:
Anti-β-Amyloid, 1-15: 1.0 - 5.0 µg/ml
It is recommended that the reagent be titrated for optimal performance for each application. - Application Notes
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For verified or reported applications for these antibodies, please see individual product datasheets.
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
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