- RTK4530 (See other available formats)
- Regulatory Status
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.Product Details
- Antibody Type
- Host Species
- Trinitrophenol + KLH
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC, ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
(PubMed link indicates BioLegend citation)
- Cervantes-Barragan L, et al. 2007. Blood 109:1131.
- Zeiser R, et al. 2007. Blood 109:2225.
- Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
- Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
- Yi H, et al. 2009. Blood 113:5819. PubMed
- Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
- Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
- Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- Gene ID
- View information about IgG2b kappa on UniProt.org
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