APC/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_APCCy7_022309.jpg
Mouse thymic epithelial stromal cell line TE-71 stained with G8.8 APC/Cyanine7
  • G8dot8_APCCy7_022309.jpg
    Mouse thymic epithelial stromal cell line TE-71 stained with G8.8 APC/Cyanine7
See APC/Cyanine7 spectral data
Cat # Size Price Save
118217 25 µg ¥20,200
118218 100 µg ¥47,700
Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
Product Citations
  1. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  2. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  3. Zwarycz B, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:1. PubMed
  4. Bretou M, et al. 2017. Sci Immunol. 2:16. PubMed
  5. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  6. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  7. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  8. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  9. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  10. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  11. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  12. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  13. Ikonomou L, et al. 2020. Nat Commun. 0.899305556. PubMed
  14. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  15. Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed
  16. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  17. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  18. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  19. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  20. Morral C, et al. 2020. Cell Stem Cell. 26(6):845-861. PubMed
  21. Mulholland D, et al. 2012. Cancer Res. 72:1878. PubMed
  22. Manzo N, et al. 2012. Am J Respir Cell Mol Biol. 47:349. PubMed
  23. Garibaldi B, et al. 2013. Am J Respir Cell Mol Biol. 48:35. PubMed
  24. Dumont-Lagacé M, et al. 2014. J Immunol. 192:2219. PubMed
  25. Zaid A, et al. 2014. Proc Natl Acad Sci U S A. 111:5307. PubMed
  26. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed
  27. Kim M, et al. 2015. J Immunol. 194:4748. PubMed
  28. St-Pierre C, et al. 2015. J Immunol. 195: 498 - 506. PubMed
  29. Dumont-Lagacé M, et al. 2015. Sci Rep. 5: 12895. PubMed
  30. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  31. Agarwal S, et al. 2015. Cell Rep. 13: 2147-2158. PubMed
  32. Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed
RRID
AB_1501158 (BioLegend Cat. No. 118217)
AB_2098648 (BioLegend Cat. No. 118218)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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