APC anti-mouse/human CD15 (SSEA-1) Antibody

Pricing & Availability
MC-480 (See other available formats)
Regulatory Status
Other Names
Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
Mouse IgM, κ
Ave. Rating
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Product Citations
Murine embryonic carcinoma cell line F9 was stained with CD15 (SSEA-1) (clone MC-480) APC (filled histogram) or mouse IgM APC Isotype control (open histogram).
  • MC-480_APC_CD15_Antibody_021419
    Murine embryonic carcinoma cell line F9 was stained with CD15 (SSEA-1) (clone MC-480) APC (filled histogram) or mouse IgM APC Isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Save
125617 25 tests ¥32,780
125618 100 tests ¥69,080

The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Host Species
Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. (IHC, IP, WB)
Product Citations
  1. Bartoccetti M, et al. 2020. Cell Reports. 30(7):2150-2169. PubMed
AB_2800604 (BioLegend Cat. No. 125617)
AB_2800605 (BioLegend Cat. No. 125618)

Antigen Details

Carbohydrate epitope, Lewis blood group antigens

Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells

Adhesion, differentiation
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.

Gene ID
14345 View all products for this Gene ID 2526 View all products for this Gene ID
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/14/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1)

  • Alexa Fluor® 488 anti-mouse/human CD15 (SSEA-1)

  • FITC anti-mouse/human CD15 (SSEA-1)

  • TotalSeq™-A0076 anti-mouse/human CD15 (SSEA-1)

  • APC anti-mouse/human CD15 (SSEA-1)

  • TotalSeq™-C0076 anti-mouse/human CD15 (SSEA-1)

  • TotalSeq™-B0076 anti-mouse/human CD15 (SSEA-1)


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