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APC anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_APC_020708
Human colon carcinoma cell line HT29 stained with 9C4 APC
  • 9C4_APC_020708
    Human colon carcinoma cell line HT29 stained with 9C4 APC
Compare all formats See APC spectral data
Cat # Size Price Save
324207 25 tests ¥32,780
324208 100 tests ¥69,080
Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Mulay A, et al. 2021. Cell Reports. 35(5):109055. PubMed
  2. Tang C, et al. 2020. Stem Cell Research. 39:101511.. PubMed
  3. Yamaguchi N, et al. 2020. eLife. 8:e52135.. PubMed
  4. Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed
  5. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  6. Puca L, et al. 2018. Nat Commun. 9:2404. PubMed
  7. Yu XX, et al. 2021. Cell Res. 31:886. PubMed
  8. Apostolou P, Ntanovasilis D, and Papasotiriou I. 2017. J Biol Res (Thessalon). 10.1186/s40709-017-0068-9. PubMed
  9. Mota STS, et al. 2019. Int J Mol Sci. 20. PubMed
  10. Yamashiro C, et al. 2020. Nat Protoc. 15:1560. PubMed
  11. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  12. Go YH, et al. 2022. Cancers (Basel). 14:. PubMed
  13. Haraguchi T, et al. 2016. Sci Rep. 6:21117. PubMed
  14. Marquez-Curtis LA, et al. 2022. PLoS One. 17:e0263005. PubMed
  15. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  16. Skrbo N, et al. 2014. PLoS One. 9:113278. PubMed
  17. Koh SB, et al. 2021. NPJ Precis Oncol. 5:85. PubMed
  18. Chua CW, et al. 2018. Elife. 7. PubMed
  19. Lo PK, et al. 2018. Elife. 7:e31334. PubMed
  20. Liu L, et al. 2018. Clin Cancer Res. 24:2370. PubMed
  21. Hornburg M, et al. 2021. Cancer Cell. . PubMed
  22. Yoshida K, et al. 2020. Nature. 578:266. PubMed
  23. Fujii M, et al. 2018. Cell Stem Cell. 23:787. PubMed
  24. Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed
  25. Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
  26. Murase Y, et al. 2020. EMBO J. 39:e104929. PubMed
  27. Balakrishnan A, et al. 2019. Clin Cancer Res. 25:7506. PubMed
  28. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  29. Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed
  30. Enge M, et al. 2017. Cell. 171:321. PubMed
  31. Mou H, et al. 2021. Cell Reports. 35(3):109011. PubMed
  32. Yamashiro C, et al. 2020. Nature Protocols. 362(6412):356-360. PubMed
  33. Grant RA, et al. 2021. Nature. 590(7847):635-641. PubMed
  34. Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
  35. Mithal A, et al. 2020. Nat Commun. 0.607638889. PubMed
  36. Wosen JE, et al. 2019. Front Immunol. 1.784722222. PubMed
  37. Kojima Y et al. 2017. Cell stem cell. 21(4):517-532 . PubMed
  38. Nian Z, et al. 2021. Clin Cancer Res. 27:6026. PubMed
  39. Xiang X, et al. 2022. Nat Commun. 13:463. PubMed
  40. Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
  41. Yan X, et al. 2019. Oncol Rep. 41:1938. PubMed
  42. Monterosso ME, et al. 2021. Sci Rep. 5118:11. PubMed
  43. Nelson L, et al. 2015. Sci Rep. 5: 17455. PubMed
  44. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  45. Yuan W, et al. 2021. Int J Biol Sci. 17:527. PubMed
  46. Menck K, et al. 2017. J Extracell Vesicles. 10.1080/20013078.2017.1340745. PubMed
  47. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  48. Ludwig LS et al. 2019. Cell. 176(6):1325-1339 . PubMed
RRID
AB_756081 (BioLegend Cat. No. 324207)
AB_756082 (BioLegend Cat. No. 324208)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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