APC anti-human CD223 (LAG-3) Antibody

Pricing & Availability
7H2C65 (See other available formats)
Regulatory Status
Other Names
CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells (PBMCs) were stained with CD8 Alexa Fluor® 488 and CD223 (Clone 7H2C65) APC (Left) or mouse IgG1, κ APC isotype control (Right).
  • 7H2C65_APC_CD223_Antibody_021218
    CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells (PBMCs) were stained with CD8 Alexa Fluor® 488 and CD223 (Clone 7H2C65) APC (Left) or mouse IgG1, κ APC isotype control (Right).
Compare all formats See APC spectral data
Cat # Size Price Save
369211 25 tests ¥31,290
369212 100 tests ¥72,870

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human LAG-3 transfected cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The staining of clone 7H2C65 cannot be blocked by clone 11C3C65, which is another anti-human CD223 (LAG-3) antibody.

Product Citations
  1. Zhang J, et al. 2022. Nature. 609:369. PubMed
  2. Cai J, et al. 2021. eLife. 10:00. PubMed
  3. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  4. Omer B, et al. 2022. Cancer Immunol Res. 10:512. PubMed
  5. Leclercq G, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Ho JY, et al. 2021. Mol Ther Methods Clin Dev. 21:237. PubMed
  7. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  8. Wang S, et al. 2021. Oncol Rep. 1.91666666666667. PubMed
AB_2728372 (BioLegend Cat. No. 369211)
AB_2728373 (BioLegend Cat. No. 369212)

Antigen Details

70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.

Activated T-cells and natural killer cells (NKs) and regulatory T cells.

Negatively regulates T-cell activation.
MHC class II molecules.
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Castelli C, et al. 2014. Oncoimmunology 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.

Gene ID
3902 View all products for this Gene ID
View information about CD223 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 02/12/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD223 (LAG-3)

  • Alexa Fluor® 647 anti-human CD223 (LAG-3)

  • PE/Cyanine7 anti-human CD223 (LAG-3)

  • PE anti-human CD223 (LAG-3)

  • FITC anti-human CD223 (LAG-3)

  • APC anti-human CD223 (LAG-3)

  • APC/Fire™ 750 anti-human CD223 (LAG-3)

  • PE/Dazzle™ 594 anti-human CD223 (LAG-3) Antibody

  • PerCP/Cyanine5.5 anti-human CD223 (LAG-3)

  • APC/Cyanine7 anti-human CD223 (LAG-3)

  • PE/Fire™ 640 anti-human CD223 (LAG-3)

  • PE/Fire™ 810 anti-human CD223 (LAG-3)


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account