- 5F4 (See other available formats)
- Regulatory Status
- Other Names
- 4-1BBL, CD137L, TNFSF9, 41BBL
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
(PubMed link indicates BioLegend citation)
- Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed
- Product Citations
AB_2561310 (BioLegend Cat. No. 311505)
AB_2561311 (BioLegend Cat. No. 311506)
- TNF superfamily, 97 kD
Antigen presenting cells, activated B cells and T cells
- T cell proliferation, cytokine production
- 4-1BB (CDw137)
- Cell Type
- Antigen-presenting cells, B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Akiba H, et al. 2000. J. Exp. Med. 191:375.
2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488.
3. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.
- Gene ID
- 8744 View all products for this Gene ID
- View information about CD137L on UniProt.org
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