Anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39300)

Pricing & Availability
6E10 (See other available formats)
Regulatory Status
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Signet Catalog# 9300-02
Signet Catalog# 9300-05
Signet Catalog# 9300-10
Covance Catalog# SIG-39300
Mouse IgG1, κ
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Product Citations
Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20 µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_6E10_ASCITES_betaAmyloid_1-16_WB_050818
    Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20 µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_6E10_ASCITES_betaAmyloid_1-16_DirectELISA_050818
    Direct ELISA of anti-β-amyloid, 1-16 (clone 6E10) antibody binding to the plate-immobilized human Aβ1-40, human Aβ1-42, rodent Aβ1-42 peptides, and recombinant human APP751 protein. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • C_6E10_ASCITES_betaAmyloid_1-16_IHCP_050818
    IHC staining of anti-β-Amyloid, 1-16 antibody (clone 6E10) on formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel)brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1:5,000 dilution of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Compare all formats
Cat # Size Price Save
803014 50 µL ¥59,010
803015 200 µL ¥207,690
803016 500 µL ¥422,520
803017 1 mL ¥708,330

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.

WB - Quality tested
Direct ELISA, IHC-P - Verified
IHC-F, EM - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1,000 to 1:20,000. For Direct ELISA, the suggested dilution of this reagent is 1:10,000 to 1:100,000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested dilution of this reagent is 1:1,000 to 1:20,000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed
  2. Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed
  3. Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed
  4. Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed
  5. Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed
  6. Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed
  7. Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed
  8. Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F)
  9. Kumar R, et al. 2016. Brain. 139:174-92 (WB)
  10. Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB)
  11. Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed
  12. Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed
  13. Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed
  14. Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed
Product Citations
  1. Jang J, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.08.127. PubMed
  2. Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed
  3. Fu W, et al. 2018. Exp Ther Med. 128:2252. PubMed
  4. Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
  5. Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed
  6. Williamson RL, et al. 2017. J Biol Chem. 292:19873. PubMed
  7. Cheng WH, et al. 2019. Alzheimers Res Ther. 11:6. PubMed
  8. Li N, et al. 2019. Neural Regen Res. 14:1037. PubMed
  9. Kordower JH, et al. 2017. Ann Neurol. 81:46. PubMed
  10. Lana E, et al. 2019. Front Mol Neurosci. 12:176. PubMed
  11. Seino S, et al. 2018. Biomed Res. 39:105. PubMed
  12. Robert J et al. 2017. eLife. 6 pii: e29595. PubMed
  13. Xiao Zhang et al. 2017. The Journal of Neuroscience. 37(19):4928-4941 . PubMed
  14. Meilandt WJ, et al. 2020. J Neurosci. 40:1956. PubMed
  15. Song JX, et al. 2020. Aging Cell. 19:e13069. PubMed
  16. Porter KN, et al. 2020. Front Aging Neurosci. 12:92. PubMed
  17. Soto-Mercado V, et al. 2020. PLoS One. 15:e0221669. PubMed
  18. Ren Z, et al. 2020. Mol Med Rep. 22:201. PubMed
  19. Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed
  20. Tanaka H, et al. 2020. Nat Commun. 11:507. PubMed
  21. Suh J, et al. 2020. Cell. 178(5):1159-1175.e17.. PubMed
  22. Shen Y, et al. 2020. Theranostics. 10:11794. PubMed
  23. Klohs J, et al. 2016. J Cereb Blood Flow Metab. 36: 1614 - 1624. PubMed
  24. Zheng Y, et al. 2012. PLoS One. 7:e39035. PubMed
  25. Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed
  26. Gowert N, et al. 2014. PLoS One. 9:e90523. PubMed
  27. Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed
  28. Chan E, et al. 2016. Sci Rep. 6: 26119. PubMed
  29. Heiss J, et al. 2016. Cereb Cortex. 10.1093/cercor/bhw188. PubMed
  30. Li T, et al. 2016. Nat Commun. 7:12082. PubMed
  31. Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed
  32. Beraldo F, et al. 2016. J Biol Chem. 291: 21945 - 21955. PubMed
  33. Zhang H, et al. 2016. J Neurosci. 36: 11837 - 11850. PubMed
  34. Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed
  35. Zhang X, et al. 2020. Sci Adv. 6:00. PubMed
  36. Dai Y, et al. 2020. Front Cell Dev Biol. 8:593659. PubMed
  37. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  38. Quartey MO, et al. 2021. Sci Rep. 0.757638889. PubMed
  39. Su Q, et al. 2021. Alzheimers Res Ther. 13:07. PubMed
  40. Benthem SD, et al. 2020. Current Biology. 30(13):2588-2601.e5. PubMed
  41. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
AB_2728527 (BioLegend Cat. No. 803014)
AB_2565328 (BioLegend Cat. No. 803015)
AB_2565329 (BioLegend Cat. No. 803016)
AB_2565327 (BioLegend Cat. No. 803017)

Antigen Details

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
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Go To Top Version: 4    Revision Date: 03/12/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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