- Poly8402 (See other available formats)
- Regulatory Status
- Other Names
- Beta-Secretase 1, Asp 2, Memapsin-2, App Beta-Secretase, Aspartyl Protease 2, Beta-Site App Cleaving Enzyme 1, Beta-Secretase 1 Precursor Variant 1, Transmembrane Aspartic Proteinase Asp2, Membrane-Associated Aspartic Protease 2, Amyloid Beta A4
Covance Catalog# PGP-624C
- Guinea Pig Polyclonal
- Ave. Rating
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- Product Citations
Processing of the β-amyloid precursor protein (βAPP) by two endopeptidases, β and γ secretases generates the amyloidogenic peptide Aβ, a major factor in the etiology of Alzheimers disease. The beta secretase is a novel membrane bound aspartyl protease and cleaves the βAPP to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase resulting in the generation and secretion of the Aβ.Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Guinea Pig
- GP45 was raised in guinea pig against a peptide sequence CLRQQHDDFADDISLLK , the C-terminal 17 amino acids of the human BACE1 and recognizes the mature and immature forms of BACE.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
WB - Quality tested
IP, ICC, IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:1,000. It is recommended that the reagent be titrated for optimal performance for each application.
The optimal working dilution should be determined for each specific assay condition.
• IP: 1:200
• IF: 1:300
- Application Notes
This antibody is effective in immunoblotting, immunoprecipitation (IP) and immunofluorescence (IF).
Clone also known as GP45.
(PubMed link indicates BioLegend citation)
- Yan R, et al. 2001. J Biol Chem. 276:36788-96.
- Product Citations
AB_2565446 (BioLegend Cat. No. 840201)
|Anti-BACE1 (CO2 terminus)||Poly8402||WB,IP,ICC,IHC|
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