- C15.6 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
The C15.6 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer, or heterodimer. The C15.6 antibody can not neutralize the bioactivity of natural or recombinant IL-12.Product Details
- Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
- Antibody Type
- Host Species
- CHO-expressed, recombinant mouse IL-12 p70
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
ELISA or ELISPOT Capture1,2,4,6,8,10: The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF™ purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: immunohistochemical staining9 of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation3, and Western blotting3.
Note: For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
- Reichmann G, et al. 1999. J. Immunol. 163:3354.
- Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
- Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
- Macatonia S, et al. 1995. J. Immunol. 154:5071.
- O'Connell PJ, et al. 2006. Blood 107:1010.
- Akilov OE, et al. 2007. J. Leukocyte Biol. 2007;10.1189/jlb.0706439.
- Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
- Huang LY, et al. 2001. J. Immunol. 167:1423.
- Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed
- Product Citations
AB_2894642 (BioLegend Cat. No. 505213)
- Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
- IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
- Cell Sources
- Dendritic cells, monocytes/macrophages, B cells, T cells
- Cell Targets
- T cells, NK cells
- IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford, CS, et al. 2003 J. Leukocyte Biol. 73:49.
- Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
- Gene ID
- 16160 View all products for this Gene ID
- View information about IL-12 p40 on UniProt.org
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