Alexa Fluor 647 anti-ZAP70 Antibody anti-ZAP70

Clone: A15114B (See other available formats)
Regulatory Status: RUO
Other Names: Zeta chain associated protein kinase, SYK related tyrosine kinase, ZAP-70
Isotype: Mouse IgG2b, κ
Ave. Rating: Submit a Review
Product Citations: publications

A15114B_A647_ZAP70_Antibody_1_010218 — Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat No. 420801), permeabilized with cold True-Phos™ Perm Buffer (Cat No. 425401), then stained with CD3 Alexa Fluor® 488 and anti-ZAP70 (clone A15114B) Alexa Fluor® 647 (top) or mouse IgG2b, κ PE (Bottom).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
691205	25 tests	¥29,920
691206	100 tests	¥76,340

Description

ZAP70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP70 have been linked to selective T cell defects. The ZAP70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-peptide surrounding ZAP70 Y319.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
RRID: AB_2721438 (BioLegend Cat. No. 691205)
AB_2721439 (BioLegend Cat. No. 691206)

Antigen Details

Structure: Protein tyrosine kinase, contains two SH2 domains. Human Zap70 has three isoforms with predicted MW of 70kD, 56kD, 36kD. Mouse Zap70 has three isoforms with predicted MW of 70kD, 36kD, 35kD.
Distribution: T cells, NK cells.
Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Interaction: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated.
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Chan AC, et al. 1992. Cell. 71:649.
2. Chan AC, et al. 1994. Science. 264:1599.
3. Arpaia E, et al. 1994. Cell. 76:947.
Gene ID: 7535 View all products for this Gene ID
UniProt: View information about ZAP70 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All ZAP70 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-ZAP70	A15114B	WB,ICFC
Alexa Fluor® 647 anti-ZAP70	A15114B	ICFC
PE anti-ZAP70	A15114B	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.