Alexa Fluor® 647 anti-mouse I-Ek/rat RT1D Antibody

Pricing & Availability
14-4-4S (See other available formats)
Regulatory Status
Other Names
I-Ek, MHC class II
Mouse (C3H.SW) IgG2a, κ
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Product Citations
C3H/He mouse splenocytes stained with 14-4-4S Alexa Fluor® 647
  • 14-4-4s_Alx647_020608
    C3H/He mouse splenocytes stained with 14-4-4S Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Save
110211 25 µg ¥15,940

The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Host Species
C3H mouse skin graft and splenocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1980. J. Immunol. 124:533.
  2. Nei U, et al. 1994. Int. Immunol. 6:61. (IP)
  3. Harton JA, et al. 1991. Immunogenetics 34:358.
  4. Roy M, et al. 1995. Eur. J. Immunol. 25:596.
  5. Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC)
  6. Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Product Citations
  1. Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
AB_493214 (BioLegend Cat. No. 110211)

Antigen Details

MHC class II

B cell and activated T cells, APCs of H-2b mice

Antigen presentation
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14969 View all products for this Gene ID
View information about I-Ek on

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Go To Top Version: 3    Revision Date: 04/21/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse I-Ek/rat RT1D

  • Alexa Fluor® 647 anti-mouse I-Ek/rat RT1D


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