- 14E6-4E6 (See other available formats)
- Regulatory Status
- Other Names
- Protein Lin-28 Homolog A, Lin-28A, CSDD1, ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
- Mouse IgG2a, κ
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- Product Citations
LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant human Lin28A from E. coli
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Red Laser (633 nm)
- Application Notes
This clone is specific for Lin28A does not recognize Lin28B.
AB_2924622 (BioLegend Cat. No. 657805)
- 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules
- Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
- EIF3S2, NCL and ZCCHC11/TUT4
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
- Antigen References
1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395.
2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474.
3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483.
4. Cho J, et al. 2012. Cell. 151:765.
5. Li N, et al. 2012. J. Biol. Chem. 287:17386.
6. Zhu H, et al. 2011. Cell 147:81.
- Gene ID
- 79727 View all products for this Gene ID
- View information about Lin28A on UniProt.org
|Purified anti-Lin28A||14E6-4E6||WB, ICC, ICFC|
|Alexa Fluor® 647 anti-Lin28A||14E6-4E6||ICFC|
|Alexa Fluor® 488 anti-Lin28A||14E6-4E6||ICFC|
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