- D8B7 (See other available formats)
- Regulatory Status
- Other Names
- Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
The spectrins are a family of widely distributed cytoskeletal proteins which are involved in actin crosslinking. Spectrin α-chain heterodimerizes with tissue-specific β-chains. It is specifically expressed in nonerythrocytic cells. The protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the gene are the cause of early infantile epileptic encephalopathy. Fodrin, a spectrin that seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.Product Details
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.
This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.
Reactivity to Drosophila was only verified with the purified format.
(PubMed link indicates BioLegend citation)
- Konieczny P, et al. 2008. J Cell Biol. 181:667-81
- Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
- Xu J, et al. 2000. J Cell Science. 113:3805-14
- De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
- Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
- Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
- Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
- Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
- Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed
AB_2820121 (BioLegend Cat. No. 803211)
AB_2820122 (BioLegend Cat. No. 803212)
- Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Presynaptic proteins, Scaffold Proteins
- Antigen References
- Pike BR, et al. 2001. J Neurochem. 78(6):1297-306.
- Weiss ES, et al. 2009. Ann Thorac Surg. 88(2):543
- Gene ID
- 6709 View all products for this Gene ID
- View information about Alpha-II Spectrin on UniProt.org
Related Pages & Pathways
Other FormatsView All Alpha-II Spectrin Reagents Request Custom Conjugation
|Purified anti-Alpha-II Spectrin||D8B7||IHC-P,ICC,WB|
|HRP anti-Alpha-II Spectrin||D8B7||WB,IHC-P|
|Biotin anti-Alpha-II Spectrin||D8B7||WB,IHC-P|
|Alexa Fluor® 647 anti-Alpha-II Spectrin||D8B7||IHC-P|
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