Alexa Fluor® 594 anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48dash1_A594_CD48_Antibody_IHCF_063016
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of CD48 (clone HM48-1) Alexa Fluor® 594 (red), 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 647 (green), and 5 µg/ml of B220 (clone RA3-6B2) Brilliant Violet 421™ (blue) at 4°C overnight. The image was captured with a 10X objective.
  • HM48dash1_A594_CD48_Antibody_IHCF_063016
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of CD48 (clone HM48-1) Alexa Fluor® 594 (red), 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 647 (green), and 5 µg/ml of B220 (clone RA3-6B2) Brilliant Violet 421™ (blue) at 4°C overnight. The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Save
103436 100 µg ¥53,000
Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
RRID
AB_2617019 (BioLegend Cat. No. 103436)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/30/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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