Alexa Fluor® 594 anti-mouse CD38 Antibody

Pricing & Availability
Clone
90 (See other available formats)
Other Names
T10
Isotype
Rat IgG2a, κ
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Product Citations
publications
90_A594_CD38_Antibody_IHCF_051419
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of CD38 (clone 90) Alexa Fluor® 594 (red), 5 µg/ml of F4/80 (clone BM8) Alexa Fluor® 647 (blue), and 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
  • 90_A594_CD38_Antibody_IHCF_051419
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of CD38 (clone 90) Alexa Fluor® 594 (red), 5 µg/ml of F4/80 (clone BM8) Alexa Fluor® 647 (blue), and 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Save
102725 100 µg ¥43,000
Description

CD38 is a 42 kD glycoprotein, also known as T10. It is an ADP-ribosyl hydrolase, expressed on B cells, NK cells, a subset of T cells, brain, muscle, and kidney. In mouse, CD38 expression is downregulated on germinal center B cells and plasma cells, whereas this is not the case for humans. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, as well as adhesion and metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 0.1 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections, and induction of B cell proliferation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Oliver AM, et al. 1997. J. Immunol. 158:1108.
  2. Howard M, et al. 1993. Science 262:1056.
RRID
AB_2566435 (BioLegend Cat. No. 102725)

Antigen Details

Structure
ADP-ribosyl hydrolase, 42 kD
Distribution

B cells, NK cells, T cell subset, brain, muscle, kidney

Function
Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor
CD31, hyaluronic acid
Cell Type
B cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Shubinsky G, et al. 1997. Immunity 7:315.
3. Cesano A, et al. 1998. J. Immunol. 160:1106.
4. Cockayne DA, et al. 1998. Blood 92:1324.

Gene ID
12494 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/05/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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