Product: Alexa Fluor® 488 anti-mouse CD8a |
Catalog No.: 100723 |
Chris Corkum, Research Assistant Memorial University of Newfoundland |
Bright Staining of Mouse Splenocytes by Flow Cytometry (Alexa Fluor® 488 anti-mouse CD8a) | |||
Overall: | Product Quality: | Ease of Use: | |
This antibody performed extremely well in staining CD8+ mouse splenocytes by flow cytometry. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | Mouse splenocytes were isolated using a cell strainer. Approximately 1x10^6 Cells were incubated with the antibody for 1 hour on ice at 1:200 dilution in a total volume of 100ul PBS+2% FCS, 1mM EDTA, 0.1% sodium azide. Cells were washed twice and fixed with 4% PFA for 20 min on ice, followed by two additional washes. |
Results Summary: | Approximately 8% of mouse splenocytes stained bright for CD8. |
Additional Notes: |
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