Application: |
Purification of tagged protein overexpressed |
Cells used: |
HEK 293T transfected with lentiviral vector |
Brief Protocol: |
Extraction of nuclear proteins Interaction 4 hours beetween about 2mg of protein with 20 ul of product Washes of resin Samples have been boiled for 10 min. at 95°C to remove tagged protein from resin. |
Results Summary: |
Tagged protein has been purified but, in several experiments with Western Blot, and also in the negative control, we see not tagged proteins. |
Additional Notes: |
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