Product: Brilliant Violet 605™ anti-mouse Ki-67 |
Catalog No.: 652413 |
Jacqueline Kimmey, Graduate Student Washington University in St. Louis |
Great Ab and Color (Brilliant Violet 605™ anti-mouse Ki-67) | |||
Overall: | Product Quality: | Ease of Use: | |
Ab works as expected, bright and clear staining. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Murine bone marrow and murine blood cells |
Brief Protocol: | Prep cells for flow, as normal. Perform surface staining and then fix in PFA. Perm with BioLegend FoxP3 / Fix Perm, as per protocol, (this worked better for me than the TrueNuclear™ buffer). Stain cells with anti-Ki67 (1:200 for 30 min, room temp). Wash in FACS buffer, analyze cells. |
Results Summary: | Clear positive and negative populations. |
Additional Notes: |
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