Product: Zombie Aqua™ Fixable Viability Kit |
Catalog No.: 423102 |
Ross Pollack, Research Technician Johns Hopkins University |
Inexpensive Quick and Easy to Use Live/Dead Stain | |||
Overall: | Product Quality: | Ease of Use: | |
This stain is inexpensive, easy to use, and the protocol is very quick. |
Experimental Design |
Application: | Differentiated Live from Dead cells to gate away from dead cells and reduce background from autoflourescent cells. |
Cells used: | Primary CD4+ Lymphocytes, HEK 293T, Jurkat |
Brief Protocol: | Wash away media, stain, add FBS, wash, fix/perm |
Results Summary: | Easily distinguishable two populations in FACS plot. |
Additional Notes: |
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Technical Service Notes |
Note that Zombie is recommended to be added prior to fixation. In addition, FBS should not be included in the staining solution as it can be bound by Zombie. |
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