Application: |
Flow cytometry |
Cells used: |
Lung tissue homogenates and bronchoalveolar lavage |
Brief Protocol: |
Product resuspended in DMSO as recommended by BioLegend. Used at 1 ul per 1 ml concentration in 500 ul per sample. Stained for 15 minutes in dark, room temperature. Assessed by flow cytometry in a multi-panel design (10 colors). |
Results Summary: |
Detected dead cells from all populations of interest, resolved completely from live populations. |
Additional Notes: |
Protocol and results completely comparable to Life Technologies Aqua dye, great reliable alternative. |
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