Product: Zombie Aqua™ Fixable Viability Kit

Catalog No.: 423101

Sergejs Berdnikovs, Research Assistant Professor Northwestern University

Zombie Aqua™ Live/Dead Stain
Overall:	Product Quality:	Ease of Use:
Assessing viability of cells by flow cytometry using Zombie Aqua dye.

Experimental Design

Application:	Flow cytometry
Cells used:	Lung tissue homogenates and bronchoalveolar lavage
Brief Protocol:	Product resuspended in DMSO as recommended by BioLegend. Used at 1 ul per 1 ml concentration in 500 ul per sample. Stained for 15 minutes in dark, room temperature. Assessed by flow cytometry in a multi-panel design (10 colors).
Results Summary:	Detected dead cells from all populations of interest, resolved completely from live populations.
Additional Notes:	Protocol and results completely comparable to Life Technologies Aqua dye, great reliable alternative.

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Publication Status This data has not been published.

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