Product: Alexa Fluor® 647 anti-mouse IL-22

Catalog No.: 516406

Mahesh Raundhal, Research Fellow Dana-Farber Cancer Institite

Alexa Fluor® 647 Stains Intracellular IL-22 Extremely Well!
Overall:	Product Quality:	Ease of Use:
Excellent antibody for IL-22 detection by flow cytometry.

Experimental Design

Application:	Intracellular flow cytometry
Cells used:	In vitro differentiated T cells
Brief Protocol:	T cells were cultured in vitro in the presence of IL-6, IL-2, IL-1b, IL-23, anti-IL-4, and anti-IFNg for 3 days and were then stimulated with Cell Stimulation Cocktail for 4 hrs. Cells were then stained with viability dye, fixed with 2% paraformaldehyde, and stained for intracellular IL-22.
Results Summary:	T cells cultured in the presence of IL-6, IL-1b, and IL-23 secrete IL-22.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

See all Reviews