Product: Alexa Fluor® 700 anti-human CD16 |
Catalog No.: 302026 |
Oliver Burton, Instructor Boston Children's Hospital |
Alexa Fluor® 700 anti-human Bright CD16 Antibody for Far Red | |||
Overall: | Product Quality: | Ease of Use: | |
Brightly stains monocytes and granulocytes in peripheral blood. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human peripheral blood |
Brief Protocol: | Whole heparinzed blood (100ul) was blocked with 5ul FcX TruStain and then stained with 5ul anti-CD16 Alexa Fluor 700®. A non-specific IgG2b conjugated to Alexa Fluor 700® was used as an isotype control. Cells were incubated for 30min at room temperature, and then stopped with BD Lyse/Fix reagent. Mononuclear cells were washed twice with FACS buffer and analyzed on an LSR Fortessa. |
Results Summary: | Clear separation of monocyte and granulocyte populations. Lymphocytes unstained. |
Additional Notes: |
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