Product: APC anti-mouse CD279 (PD-1)

Catalog No.: 135210

Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

Anti-mouse CD279 (PD-1) APC for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
APC anti-mouse CD279 (PD-1) is a good option.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse splenocytes
Brief Protocol:	Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti-PD-1 APC (1:100). Incubate 15min at 4°C in the dark. Wash twice with PBS/BSA, resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells.
Results Summary:	Some CD4 and fewer CD8 T cells express PD-1 in the spleen of naïve mice.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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