Product: PE anti-mouse CD1d (CD1.1, Ly-38)

Catalog No.: 123510

J Lykken, Dr. Duke University

Clearly Marks CD1d Expression Among Mouse Lymphocytes
Overall:	Product Quality:	Ease of Use:
Mouse splenic B cell CD1d expression was easily visualized with this antibody.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse Splenocytes
Brief Protocol:	Isolate splenocytes. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer. Stain with isotype control or mouse CD1d PE antibody (1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL FACS buffer containing 1.5% paraformaldehyde.
Results Summary:	Mouse splenic B cell CD1d expression was easily visualized.
Additional Notes:	Mouse splenic B cell CD1d expression directly ex vivo (left panel) or following 5 hour LPS stimulation (right panel) was used to phenotype spleen B cell populations relative to CD5 expression.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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