Product: PE anti-mouse CD45

Catalog No.: 103105

J Lykken, Dr. Duke University

Brightly Labels CD45 Expression Among Mouse (C57BL/6) Leukocytes (PE)
Overall:	Product Quality:	Ease of Use:
CD45-expressing leukocytes were clearly labeled among total splenocytes.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse Splenocytes
Brief Protocol:	Isolate splenocytes. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer. Stain with isotype control or mouse CD45 antibody (1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL FACS buffer containing 1.5% paraformaldehyde.
Results Summary:	CD45 expression among mouse leukocytes was brightly distinguished.
Additional Notes:	Plots show CD45 expression among mouse splenocytes (middle panel, shaded histogram in right panel) relative to isotype control (left panel, empty histogram in right panel).

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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