Product: Brilliant Violet 421™ anti-mouse CD8a

Catalog No.: 100737

Kevin Meng, Mr. Stanford University

Review of CD8a Brilliant Violet 421™ in Detecting CD8+ T Cells in Mouse OT-I Splenocytes
Overall:	Product Quality:	Ease of Use:
Successfully labels CD8+ T cells in mouse spleen.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse splenocytes
Brief Protocol:	After 6-day stimulation of OT-I splenocytes with Ova peptide and IL-2: Wash splenocytes with FACS buffer. Incubate cells with Fc block (1:100 dilution). Add CD8a Brilliant Violet 421™ directly to the cells (1:100 final concentration) and incubate for 30 min on ice. Wash 3x with FACS buffer. Complete FACS analysis.
Results Summary:	Labels CD8+ T cells.
Additional Notes:	Detecting CD8+ T cells in stimulated OT-I splenocytes.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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