Product: Brilliant Violet 605™ anti-mouse/human CD45R/B220 |
Catalog No.: 103243 |
Kevin Meng, Mr. Stanford University |
Review of CD45R/B220 Brilliant Violet 605™ Antibody in Mouse Spleen | |||
Overall: | Product Quality: | Ease of Use: | |
Successfully detects B cells in the mouse spleen with little background. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Mouse spleen |
Brief Protocol: | Extract mouse splenocytes Incubate with Fc block (1:50) for 5-10 min on ice. Add CD45R/B220 Brilliant Violet 605™ antibody directly onto the cells (1:100 final concentration). Incubate on ice for 20-30 min. Wash with FACS buffer 3x. Complete FACS analysis. |
Results Summary: | Detects B cells in the mouse spleen with little background. |
Additional Notes: | Staining B cells in mouse spleen to be used as a negative gate. |
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