Product: PE/Dazzle™ 594 anti-human CD294 (CRTH2) |
Catalog No.: 350125 |
Romy Hackbusch, Miss University Medical Center Hamburg-Eppendorf |
Anti-human CD294 (CRTH2) - PE/Dazzle™ Texas Red | |||
Overall: | Product Quality: | Ease of Use: | |
Easy detection of CRTH2-positive cells within a CD4-population. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human whole blood |
Brief Protocol: | 1. 80 µl EDTA blood plus 20 µl antibody cocktail (in FACS Buffer), incubate for 30 min at RT. 2. Lysis of erythrocytes by adding 1 ml of Lysis Buffer, incubate for 10 min at RT. 3. Wash two times with PBS; centrifuge at 400 x g for 5 min, trash supernatant. 4. Resuspend cells in 200-300 µl FACS Buffer, FACS analysis. 5. CD294-PE/Texas Red was titrated and applied at a final dilution of 1:50. |
Results Summary: | Usually PE/Texas Red is very bright and can be used highly diluted, but from this antibody you need quite a bit. |
Additional Notes: | Gating strategy: Lymphocytes > single cells > CD4+ > CD294+ |
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