- Clone
- TUJ1 (See other available formats)
- Other Names
- CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
ICC staining of Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3) (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum. The cells were then stained with 1 µg/ml of the primary antibody for three hours at room temperature. Nuclei were counterstained with Hoechst 33342. The image was captured with a 60X objective.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 801207 | 25 µg | ¥27,000 | |
| 801208 | 100 µg | ¥63,000 |
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
Product DetailsProduct Details
- Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against microtubules derived from rat brain.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing no preservatives solution.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, and Western blotting8.
This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8. -
Application References
(PubMed link indicates BioLegend citation) -
- Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
- Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
- Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
- Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
- Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
- Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
- Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
- Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
- Product Citations
- RRID
-
AB_2650635 (BioLegend Cat. No. 801207)
AB_2650636 (BioLegend Cat. No. 801208)
- Disclaimer
-
Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.
Antigen Details
- Structure
- Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
- Distribution
-
Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus. - Function
- Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
- Interaction
- Alpha tubulin, kinesin and dynein.
- Cell Type
- Mature Neurons, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Microtubules
- Antigen References
-
1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed - Gene ID
- 10381 View all products for this Gene ID
- UniProt
- View information about Tubulin beta 3 (TUBB3) on UniProt.org
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