BioLegend's LEGENDplex™ bead-based immunoassays quantify multiple soluble analytes simultaneously in biological samples using a flow cytometer. The assay utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.

LEGENDplex™ kits are provided with predefined panels, ranging from 3 to 13 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system. Custom options are also available.

Each kit provides sufficient reagents for at least 100 tests. The assay can be performed in a 96-well filter plate or v-bottom plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate, or they can be performed in FACS tubes, where duplicate titrations may not be necessary.

Software is downloaded for free and each kit comes with a dongle key required for use.

LEGENDplex™ Predefined Panels

The LEGENDplex™ panels cover a wide range of soluble markers for immunology, growth factors, and metabolism. Currently, reactivities include human, mouse, rat, and non-human primate species (Rhesus and Cynomolgus). Panels are carefully selected for important biomarkers that are often analyzed together in a set.

LEGENDplex™ Mix and Match

Our Mix and Match system is more suited for researchers requiring flexibility in the in their choice of analytes. Instead of ordering all 13 specificities in a predefined panel, you can choose any subset within a panel, even a single analyte if desired. The system is easy to use and cost-effective. Just order the analyte capture beads, the appropriate lyophilized standard, the appropriate detection antibodies, and the appropriate buffer set.

Mix and Match kits must be ordered using the LEGENDplex™ page.

BioLegend provides an extensive selection of purified and conjugated antibodies against human, mouse, and rat soluble proteins for use in a variety of applications, such as ELISA, ELISPOT, Flow Cytometry, Western Blot, and more. Browse the categories below:

Useful Tools

For unparalleled performance in soluble protein quantitation by ELISA, BioLegend provides three options for kits and sets: LEGEND MAX™ kits with pre-coated plates, ELISA MAX™ Deluxe and ELISA MAX™ Standard sets. In addition, we also provide optimized antibody pairs for ELISA detection and recombinant protein standards, sold separately as single reagents.

LEGEND MAX™ Kits contain a complete set of pre-coated plates and reagents necessary for your ELISA experiment. This is the optimal kit for those with a limited familiarity with ELISAs as well as for those who are fully knowledgeable with the technique but short on time.

ELISA MAX™ Deluxe and Standard Sets are a cost effective choice for intermediate and experienced ELISA users. If you are already running many different ELISAs and use your own plates and buffers, we recommend the Standard Set. On the other hand, our Deluxe set includes uncoated plates as well as most of the buffers. See the table below for all the components in each set.

Components LEGEND MAX™
Deluxe Set
Standard Set
Pre-coated 96-well stripwell plate    
Pre-titrated Capture Antibody  
Pre-titrated Detection Antibody
Recombinant Protein Standard
Coating Buffer    
Wash Buffer    
Assay Buffer/Assay Diluent/Matrix  
Substrate/TMB Solution  
Stop Solution    
Plate Sealers    
Assay Time 4 hours Overnight + 6 hours Overnight + 6 hours
Experience Level Beginner - Advanced Intermediate - Advanced Intermediate - Advanced

Having the correct standards is critical for the accuracy and dependability of an ELISA assay. BioLegend provides lyophilized recombinant protein standards, optimized for use with our ELISA pairs. Browse products by species below:

ELISA Standards



To view all our available recombinant proteins, including those for bioassays and animal-free formats, visit our Recombinant Proteins page.

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