Phosphorylation of proteins is an important post-translational modification that plays a critical role in mediating physiological processes of the cell, including signal transduction pathways, cell cycle, growth, and death, among others. The process requires protein kinases, which mediate the addition of a phosphate group to the target protein by hydrolysis of ATP. At BioLegend, we have developed True-Phos™ Perm Buffer, which is specifically designed to help detect our highly sensitive phosphorylation-specific antibodies to study this regulatory process by flow cytometry.
Brilliant Violet 421™ Anti-STAT3 Phospho (Tyr705)
Human whole blood unstimulated (left panel) or stimulated with IL-6 for 15 minutes (right panel), were treated with RBC Lysis/Fixation Solution (10X), permeabilized with True-Phos™ Perm Buffer, and stained with CD3 APC and STAT3 Phospho (Tyr705) Brilliant Violet 421™.
Alexa Fluor® 647 Anti-Histone H3 Phospho (Ser10)
HeLa cells were treated with Nocodozole for 24 hours (right panel) or untreated (left panel), fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then stained with DAPI and Histone H3 Phospho (Ser10) Alexa Fluor® 647.

Phospho-Protein and Nuclear Factor Specific Antibodies

Buffers
Description
True-Phos™ Perm Buffer
Antibodies
Description
ATM Phospho (Ser1981)
Aurora A (Aurora 2) Phospho (Thr288)
Aurora B Phospho (Thr232)
B-Raf Phospho (Thr598/Ser601)
C2H2 zinc finger protein phospho linker region (HpTGEKP)
CAK (cdk7) Phospho (Ser164/Thr170)
Cyclin B1
ERK1/2 Phospho (Thr202/Tyr204)
H2A.X-Phosphorylated (Ser139)
Histone H3 Phospho (Ser10)
Histone H3-Phosphorylated (Ser28)
IRF6
PLK-1 Phospho (Thr210)
STAT3 Phospho (Tyr705)
STAT6 Phospho (Tyr641)
TIF1β (KAP-1, TRIM28) Phospho (Ser473)
TSC2 Phospho (Ser664)
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