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We developed our new Cyto-Fast™ Fix/Perm Buffer Set after rigorous in-house testing to determine optimal buffer formulations for cytokine staining. Advantages of the Cyto-Fast™ Fix/Perm Buffer set include:

  • Improved signal-to-noise for staining of cytokines and other intracellular proteins like Granzyme B and Perforin.
  • Maintains signal intensity of surface antibody stains.
  • Fixation and permeabilization buffers are sold and quality tested as a set.
Description: Size Cat. No.
300 tests 426803

Samples Available! Contact sales@biolegend.com for more information.

PMA + ionomycin stimulated (3 hours) human peripheral blood lymphocytes (in the presence of monensin) were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set. Cells were then stained with antibodies as indicated.
If you’re looking for a way to reliably quantify soluble cytokines, chemokines, or biomarkers in your sample, try one of our ELISA kits.

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