Veri-Cells™ PBMC

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Other Names
Flow cytometry control cells
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Product Citations
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VeriCells_PBMC_FC_1_092815
Veri-Cells™ PBMC were stained with anti-human CD19 (clone HIB19) PerCP/Cy5.5, CD16 (clone 3G8) FITC, and CD56 (clone HCD56) FITC (top) or CD4 Brilliant Violet™ 510 and FOXP3 (clone 206D) PE (bottom).
  • VeriCells_PBMC_FC_1_092815
    Veri-Cells™ PBMC were stained with anti-human CD19 (clone HIB19) PerCP/Cy5.5, CD16 (clone 3G8) FITC, and CD56 (clone HCD56) FITC (top) or CD4 Brilliant Violet™ 510 and FOXP3 (clone 206D) PE (bottom).
  • VeriCells_PBMC_FC_2_092815
Cat # Size Price Quantity Avail. Save
425003 10 tests 120€
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425001 25 tests 200€
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425004 50 tests 400€
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425002 100 tests 720€
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Description

The reconstituted lyophilized human PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for stability studies to avoid donor variability. These cells have been verified to work with commonly tested cell markers such as CD3, CD4, CD8, CD19, CD20, CD56/16, CD14, CD25, CD69, Granzyme B, Perforin, FOXP3, and Helios. View all 150 markers verified by LEGENDScreen™.
 

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative, using FDA approved methods, for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus. Biological tests are not 100% accurate. Use standard precautions when handling and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat. No. 425001
1 vial of Veri-Cells™ PBMC (> 5 x 106 cells)
1 vial of 1.5 Veri-Cells™ Buffer A


For Cat. No. 425002
4 vials of Veri-Cells™ PBMC (> 5 x 106 cells per vial)
4 vials of 1.5 ml Veri-Cells™ Buffer A each

For Cat. No. 425003
2 vials of Veri-Cells™ PBMC (> 1 x 106 cells per vial)
1 vial of 700 µl Veri-Cells™ Buffer A each

For Cat. No. 425004
10 vials of Veri-Cells™ PBMC (> 1 x 106 cells per vial)
5 vials of 700 µl Veri-Cells™ Buffer A each

Product Details

Formulation
Cat. Nos. 425003 and 425004: ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Cat. Nos. 425001 and 425002: ≥ 5 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Preparation
The Veri-Cells™ PBMC Kit is prepared from lyophilized human PBMC and a vial of Veri-Cells™ Buffer A.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. This product is guaranteed for a minimum of 6 months, or until the expiration date stated in the COA, when properly stored. Contact BioLegend Technical Support for information on the current lots.
Application

FC - Quality tested
ICFC - Validated

Recommended Usage

50 µl per 5 tests (after reconstitution with 325 µl)
50 µl per 25 tests (after reconstitution with 1.3 ml)

Note: Veri-Cells™ Buffer A may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

The Veri-Cells™ PBMC Kit can be used as positive controls for surface immunofluorescent staining and multi-color flow cytometry assays using the BioLegend surface staining protocol. It can also be used for the detection of intracellular molecules such as Granzyme B, Perforin, Foxp3 and Helios using BioLegend’s Fixation Buffer and Intracellular Staining Permeabilization Wash Buffer (10X) or the True Nuclear™ Transcription Factor Buffer Set. For best results, Cell Staining Buffer is recommended for any washing steps. Product performance specifications are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.



Protocol for using Veri-Cells™ PBMC:

1. Remove a vial of Veri-Cell™ PBMC from the refrigerator and warm to room temperature for 5 minutes.
2. Add 1.3ml of the Veri-Cell™ Buffer A to the cell pellet, replace the rubber stopper and mix by inversion for 5 seconds. Allow at least 5 minutes for the cells to rehydrate prior to staining.
3. Aliquot the required amount of cells into tubes. Stain with antibodies using our Cell Surface Immunofluorescence Staining Protocol, Intracellular Flow Cytometry Staining Protocol. The cells are ready for acquisition.

Note: To monitor non-specific staining, we recommend using appropriate isotype controls.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ PBMC.  The antibodies used for testing were the following: CD56 PE (clone 5.1H11), CD16 PE (clone 3G8), CD19 APC (clone HIB19), CD3 FITC (clone UCHT1), CD4 PE/Cy7 (clone RPA-T4), CD8 APC/Cy7 (clone RPA-T8).  Percent positive is based on gates set on unstained cells. The Certificate of Analysis specific values are provided only as a reference. Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Additional Product Notes

View more applications data using this product to characterize their use as a control in flow cytometry assays and longitudinal studies in our  Scientific Poster Library.

Product Citations
  1. Sun Y, et al. 2016. Bioanalysis. 8(23):2399-2413. PubMed
  2. Haredy, et al. 2019. Vaccine. 5225:5232. PubMed
Publication Library

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

Which markers can be stained?

160+ known markers have been verified - please view markers verified by LEGENDScreen™ or contact tech@biolegend.com for a complete list.

Go To Top Version: 6    Revision Date: 08/14/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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