Veri-Cells™ CD4-Low PBMC

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Other Names
Flow cytometry control cells
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Veri_Cells_CD4_Low_PBMC_1_062116
Veri-Cells™ CD4-Low PBMC were stained with CD3 FITC and CD4 PE/Cy7. The bottom plot is gated on the lymphocyte population.
  • Veri_Cells_CD4_Low_PBMC_1_062116
    Veri-Cells™ CD4-Low PBMC were stained with CD3 FITC and CD4 PE/Cy7. The bottom plot is gated on the lymphocyte population.
  • Veri_Cells_CD4_Low_PBMC_2_062116
Cat # Size Price Quantity Avail. Save
425601 25 tests (1 vial) 296€
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425602 100 tests (4 vials) 1.064€
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Description

The reconstituted lyophilized human PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for stability studies to avoid donor variability. View 150 markers verified by LEGENDScreen™.

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative, using FDA approved methods, for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus. Biological tests are not 100% accurate. Use standard precautions when handling and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 425601
1 vial of lyophilized Veri-Cells™ CD4-Low PBMC (4-6 x 106 cells)
1 vial containing 1.5 ml of Veri-Cells™ Reconstitution Buffer A


For Cat# 425602
4 vials of lyophilized Veri-Cells™ CD4-Low PBMC (4-6 x 106 cells per vial)
4 vials containing 1.5 ml of Veri-Cells™ Buffer A each

Product Details

Formulation
Each vial of Veri-Cells™ CD4-Low PBMC contains 4-6 x 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Preparation
The Veri-Cells™ CD4-Low PBMC Kit is prepared from lyophilized human peripheral blood mononuclear cells and a vial of reconstitution buffer.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. This product is guaranteed for a minimum of 6 months, or until the expiration date stated in the COA, when properly stored. Contact BioLegend Technical Support for information on the current lots.
Application

FC - Quality tested
ICFC - Validated

Recommended Usage

50 µl per test (after reconstitution with 1.3 ml)

Note: Veri-Cells™ Reconstitution Buffer A may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

The Veri-Cells™ CD4-Low PBMC Kit can be used as positive controls for surface and intracellular immunofluorescent staining and multi-color flow cytometry assays. Assay values for CD3+CD4+ cells will be provided for each lot produced.



Protocol for using Veri-Cells™ CD4-Low PBMC:

  1. Remove a vial of lyophilized cells from the refrigerator and warm to room temperature for five minutes.
  2. Add 1.3 ml of the Veri-Cells™ Reconstitution Buffer A (provided) to the cell pellet, replace the rubber stopper, and mix by inversion for five seconds. Allow at least five minutes for the cells to rehydrate prior to staining.
  3. Aliquot the required amount (we recommend using 50 µl) of cells into tubes. Stain with antibodies using our recommended Cell Surface Immunofluorescence Staining Protocol, Intracellular Flow Cytometry Staining Protocol, or equivalent. The cells are ready for acquisition.

Note: To monitor non-specific staining, we recommend using appropriate isotype controls.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ CD4-Low PBMC. The antibodies used for testing were the following: CD3 FITC (clone UCHT1) and CD4 PE/Cy7 (clone RPA-T4).  Percent positive is based on gates set on unstained cells. The Certificate of Analysis specific values are provided only as a reference. Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

Which markers can be stained?

160+ known markers have been verified - please view markers verified by LEGENDScreen™ or contact tech@biolegend.com for a complete list.

Go To Top Version: 2    Revision Date: 12/15/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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