- GHI/75 (See other available formats)
- V B032
- Other Names
- ILT2, LIR-1, LILRB1, CD85
- Mouse IgG2b, κ
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- Product Citations
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CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). CD85j is the 110kD member, known as ILT2, LIR1, or LILRB1, and MIR7. ILT2 structurally has four Ig domains and contains ITIMs in its cytoplasmic tail that provide inhibitory signals by recruiting SHP-1. ILT2 is found on the surface of B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells. The ligands of ILT2 include a broad range of HLA-A, -B molecules, some HLA-C and HLA-G molecules, and the human cytomegalovirus UL18 protein.Product Details
- Antibody Type
- Host Species
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
FA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported application: Block HLA-G induced TGF-ß1 production.
(PubMed link indicates BioLegend citation)
- Pulford K, et al. 1991. Clin. Exp. Immunol. 85:429
- Mclntire RH, et al. 2004. J. Leukoc. Biol. 76:1220
AB_2814223 (BioLegend Cat. No. 333721)
AB_2814224 (BioLegend Cat. No. 333722)
- ILT/LIR family, Ig superfamily, 110 kD
B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells.
- Inhibitory receptor for MHC class I
- HLA-A, -B, some HLA-C and G molecules, human cytomegalovirus UL18 protein
- Cell Type
- B cells, Dendritic cells, Monocytes, NK cells, Plasma cells, T cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006
- Gene ID
- 10859 View all products for this Gene ID
- View information about CD85j on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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