Interleukin-1 is a key mediator of inflammation, with pleiotropic effects on several cells and signaling pathways. The activity defined as IL-1 reflects the function of 2 molecules, IL-1α and IL-1β. IL1A encodes IL-1α, which is cell-bound, and IL1B encodes IL-1β, a secreted cytokine (1). IL-1α and IL-1β are synthesized as 31-kD precursors and are processed by proteases to their mature 17-kD forms. IL-1β-converting enzyme cleaves the inactive IL-1β Precursor and ProIL-1α is processed by calpain (4). IL-1β is a tumor-promoting cytokine, and it enhances tumor metastasis and angiogenesis. IL-1β is able to facilitate tumor progression in murine models of lung cancer. Upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor (5).Product Details
- Mouse IL-1β, amino acids Val118-Ser269 (Accession # NM_008361) was expressed in E. coli.
- Molecular Mass
- The 152 amino acid recombinant protein has a predicted molecular mass of 17,394 Da. The DTT-reduced and the non-reduced protein migrate at approximately 19kDa by SDS-PAGE. The N-terminal amino acid is Valine.
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Mouse IL-1β induces the proliferation of D10.G4.1 mouse helper lymphocytes. The ED50 = 2 - 10 pg/ml.
- Recommended Usage
Use when high specific biological activity is required.
- Application Notes
This IL-1β protein is biologically active, and can be used for in vitro assays
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
(PubMed link indicates BioLegend citation)
- OÆSullivan BJ, et al. 2006. J. Immunol. 176:7278. PubMed
- Product Citations
IL-1β is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells.
- Type I and II IL-1 receptors (IL-1RI and IL-1RII)
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Antigen References
1. Johnsen AK, et al. Arthritis Rheum 58:1947-1957 2008.
2. Brinster C and Shevach EM J. Leukoc. Biol. 84:480-487 2008.
3. O'Sullivan BJ, et al. J. Immunol. 176:7278-7287 2006.
4. Nazarenko I, et al. Neoplasia 10:549-562 2008.
5. Boost KA, et al. BMC Cancer 8:265 2008.
- Gene ID
- 16176 View all products for this Gene ID
- View information about IL-1beta on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.