Recombinant Human IFN-γ (ELISA Std.)

Pricing & Availability
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF),
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570209 4 pack 128€
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Recombinant human IFN-γ is a 16.7 kD protein containing 143 amino acids.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human IFN-γ, Gln 24 - Gln 166 - Accession Number NM_000619, was expressed in E. coli.
Molecular Mass
The 144 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,907 Da. The DTT-reduced protein and the non-reduced protein migrate at approximately 17 kD by SDS-PAGE.
Purity
Purity is >98% as determined by SDS PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant human IFN-γ at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000 pg/ml to 8 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IFN-γ protein is useful as a standard for a human IFN-γ sandwich ELISA, using unlabeled NIB42 antibody (catalog #502402) or unlabeled MD-1 antibody (catalog #507502) for capture and biotinylated 4S.B3 antibody (catalog #502504) for detection.

Antigen Details

Structure
Cytokine; dimer
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 02/03/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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