Recombinant Human FN-1 (carrier-free)

Pricing & Availability
Other Names
Fibronectin, FN, FN1, Cold-insoluble globulin, CIG, Large external transformation-sensitive protein, LETS
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Product Citations
publications
Human_FN-1_RECOM_CF_1_082118
Human FN-1 supports the adhesion of L929 cells in a dose dependent manner. ED50 for this effect is 15 – 60 ng/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Human_FN-1_RECOM_CF_1_082118
    Human FN-1 supports the adhesion of L929 cells in a dose dependent manner. ED50 for this effect is 15 – 60 ng/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Human_FN-1_RECOM_CF_2_082118
    Stability testing for Human FN-1. Human FN-1 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for its ability to support the adhesion of L929 cells.
Cat # Size Price Quantity Avail. Save
775306 100 µg 200€
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775308 500 µg 720€
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Description

Fibronectin (FN) is an adhesive glycoprotein found as insoluble components of the extracellular matrix (ECM) or as soluble dimeric forms in plasma.  Fibronectin binds chondroitin sulfate, collagen, fibrin, heparin, and many integrin receptors through which it mediates multiple cellular signaling pathways. Conversion of soluble fibronectin to fibronectin fibrils is initiated by binding to cell surface integrins. Fibronectin is involved in cell adhesion, cell motility, wound healing and maintenance of cell morphology. It also participates in osteoblast compaction and is essential for osteoblast mineralization. Fibronectin is thought to play a role in platelet thrombus formation and other diseases associated with thrombosis including atherosclerosis and cardiac repair following a myocardial infarct. Fibronectin plays an important role in coordinating interaction between the ECM and cancer cells in tumors. Its upregulation enhances cancer cell survival, proliferation, and invasion. Fibronectin monomers consist of three types of homologous repeats, type I, II and III domains. A fragment from the first type III repeat, known as Anastellin, binds to fibronectin and induces fibril formation named superfibronectin. Superfibronectin exhibits enhanced adhesive properties and inhibits tumor growth, angiogenesis and metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human FN-1, amino acids Gln32-Pro1991 (Accession # BC117176) with a C-terminal 8His-GGQ tag was expressed in 293E cells.
Molecular Mass
The 1973 amino acid recombinant protein has a predicted molecular mass of approximately 217 kD. The DTT-reduced and non-reduced protein protein migrates at approximately 180 – 220 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
ED50= 15 - 60 ng/mL as measured by the ability of immobilized protein to support the adhesion of L929 cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin.
Interaction
Ubiquitous
Ligand/Receptor
Cell surface, collagen, fibrin, heparin, DNA, and actin.
Bioactivity
Supports the adhesion of L929 cells.
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience Cell Markers
Gene ID
2335 View all products for this Gene ID
UniProt
View information about FN-1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 08/21/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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