Recombinant Human CXCL10 (IP-10) (ELISA Std.)

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Other Names
Interferon gamma-inducible protein 10 (IP-10), Interferon gamma induced factor, gIP-10, IFI10, INP10
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Product Citations
publications
CXCL10_Human_Antibody_ELISA_121713
  • CXCL10_Human_Antibody_ELISA_121713
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573509 4 pack 128€
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Description

CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11.  CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ. CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many human inflammatory diseases, including rheumatoid arthritis, type I diabetes, multiple sclerosis, atherosclerosis, allograft rejection, and others.     

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human CXCL10, amino acids Val22-Pro98 (Accession# NM_001565) was expressed in E. coli.
Molecular Mass
The 78 amino acid recombinant protein has a predicted molecular mass of approximately 8.7 kD. The DTT-reduced and non-reduced protein migrate at approximately 10 kD by SDS-PAGE. The N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 31.3 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This CXCL10 (IP-10) protein is useful as a standard for a human CXCL10 (IP-10) sandwich ELISA, using unlabeled J036G3 antibody (Cat. No. 524401) for capture and biotinylated Poly5164 antibody (Cat. No. 519403) for detection.

Application References

(PubMed link indicates BioLegend citation)
  1. Ramirez LA, et al. 2014. J Leukoc Biol. 96:1055. PubMed
Product Citations
  1. Ramirez L, et al. 2014. J Leukoc Biol. 96:1055. PubMed
Publication Library

Antigen Details

Structure
Chemokine
Distribution

Interferon gamma-stimulated keratinocytes, monocytes, macrophages, fibroblasts, endothelial, T cells.

Function
CXCL10 chemoattracts CD4, Th1, CD8, NK, and NKT cells. CXCL10 is induced by inflammatory cytokines, and NH2-terminal cleavage of CXCL10 by DPP-IV/CD26 impairs its chemoattracting capacity and CXCR3 signaling.
Interaction
Activated T cells, Th1 cells, regulatory T cells, NK cells, NKT cells, endothelial cells, fibroblasts, endothelial cells.
Ligand/Receptor
CXCR3
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Luster AD, et al. 1985. Nature 315:672.
2. Loos T, et al. 2008. Blood 112:2648.
3. Crawford MA, et al. 2009. Infect. Immun. 77:1664.
4. Campanella GS, et al. 2010. PLoS One 5:e12700.
5. Hoerning A. 2011. Eur. J. Immunol. 41:2291.

Gene ID
3627 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/29/2016

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