Recombinant Human CTLA-4 (ELISA Std.)

Pricing & Availability
Other Names
Cytotoxic T-Lymphocyte Antigen 4 (CTLA4)
Ave. Rating
Submit a Review
Product Citations
publications
CD152_Human_ELISA_012014
  • CD152_Human_ELISA_012014
Cat # Size Price Quantity Avail. Save
591909 4 pack 128€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein that plays an important regulatory role in the immune system. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases.

An identified novel transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only one of thirty apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Accession# Q6GR94
Molecular Mass
40 kD (monomer)
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
<1.0 EU per 1 µg of the protein by the LAL method.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 2500 pg/mL to 39 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This CTLA-4 protein is useful as a standard for a human CTLA-4 sandwich ELISA, using unlabeled A3.6B10.G1 antibody (Cat. No. 525401) for capture and biotinylated L3D10 antibody (Cat. No. 349911) for detection.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Activated T cells and B cells

Function
Negative regulator of T cell activation
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86)
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Soluble Receptors, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.

Gene ID
NA
UniProt
View information about CTLA-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account