- Other Names
- Cytotoxic T-Lymphocyte Antigen 4 (CTLA4)
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CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein that plays an important regulatory role in the immune system. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases.
An identified novel transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only one of thirty apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.
- Accession# Q6GR94
- Molecular Mass
- 40 kD (monomer)
- >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
- Endotoxin Level
- <1.0 EU per 1 µg of the protein by the LAL method.
- Storage & Handling
- Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
ELISA - Quality tested
- Recommended Usage
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 2500 pg/mL to 39 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This CTLA-4 protein is useful as a standard for a human CTLA-4 sandwich ELISA, using unlabeled A3.6B10.G1 antibody (Cat. No. 525401) for capture and biotinylated L3D10 antibody (Cat. No. 349911) for detection.
- Disulfide-linked homodimer
Activated T cells and B cells
- Negative regulator of T cell activation
- B7-1 (CD80), B7-2 (CD86)
- Cell Type
- B cells, Dendritic cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- Soluble Receptors, CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.
- Gene ID
- View information about CTLA-4 on UniProt.org