Recombinant Human CD6 (carrier-free)

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Other Names
T-cell differentiation antigen CD6, CD6 antigen, T12, TP120
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Human_CD6_RECOM_CF_100917
Immobilized recombinant human CD6 supports the adhesion of Jurkat cells. ED50 for this effect is 0.15 - 0.6 µg/mL. The Deep Blue Cell Viability™ Kit (Cat # 424702) was used measure the number of adherent cells.
  • Human_CD6_RECOM_CF_100917
    Immobilized recombinant human CD6 supports the adhesion of Jurkat cells. ED50 for this effect is 0.15 - 0.6 µg/mL. The Deep Blue Cell Viability™ Kit (Cat # 424702) was used measure the number of adherent cells.
  • Human_CD6_RECOM_stability_100917
    Human CD6 was aliquoted in 20 mM Tris, 150 mM NaCl, pH 7.2 at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to support the adhesion of Jurkat cells.
Cat # Size Price Quantity Avail. Save
769302 10 µg 96€
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769304 25 µg 184€
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769306 100 µg 496€
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769308 500 µg 1.760€
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Description

T-cell differentiation antigen CD6 is a type 1 transmembrane cell adhesion molecule that is a member of the scavenger receptor cysteine rich (SRCR) family of proteins.  CD6 is composed of three scavenger receptor cysteine-rich (SRCR) extracellular domains, a transmembrane region, and a long cytoplasmic tail without intrinsic catalytic activity.  It is expressed on peripheral blood T lymphocytes, thymocytes, subset of NK and B cells, and some hematopoietic cell precursors and brain cells.  Soluble form of CD6 is detected in blood serum.   CD6 has two ligands, CD166 (ALCAM) and CD318. It plays an important role in the stabilization and maturation of immunological synapses by interacting with ALCAM.  Domain 3 of CD6 binds to ALCAM via the N-terminal domain while CD318 interacts with domain 1 of CD6. CD6 plays a role in cell to cell contacts, regulating T-cell responses via CD6-ALCAM interaction, as well as functioning as a costimulatory molecule promoting T-cell activation and T-cell proliferation by its involvement in the TCR/CD3 signaling cascade complex.  CD6 has been shown to have a high binding affinity to highly conserved bacterial components such as lipopolylsaccharides (LPS) and Lipoteichoic acid (LTA), which allows it to help regulate inflammatory responses and secretion of cytokines in response to bacterial toxins.  CD6 is also a popular target for the development of new therapeutic methods for autoimmune diseases such as multiple sclerosis due to its involvement in T-cell proliferation and activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CD6, amino acids His18-Glu398 (Accession # BC033755) with a C-terminal human IgG1 Fc and 6-His tag was expressed in CHO cells.
Molecular Mass
The 626 amino acid recombinant protein has a predicted molecular mass of approximately 68.3 kDa. The DTT-reduced protein migrates at approximately 90 - 100 kDa and and non-reduced protein migrates at approximately 190 kDa by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>90%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50= 0.15 - 0.6 µg/mL as measured by the ability of immobilized protein to support the adhesion of Jurkat cells. The Deep Blue Cell Viability™ Kit (Cat # 424702) was used measure the number of adherent cells.
Application

Bioassay

Application Notes

Marketing note:

Adhesion molecule that plays an important role in the stabilization and maturation of immunological synapses via interaction with ALCAM.


BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Peripheral blood T lymphocytes, thymocytes, subset of NK and B cells, and some hematopoietic cell precursors and brain cells.

Function
Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166.
Interaction
T cell
Ligand/Receptor
CD166, CD318
Bioactivity
Measured by its ability to support the adhesion of Jurkat cells.
Biology Area
Immunology
Molecular Family
CD Molecules, Soluble Receptors
Antigen References

1. Bowen MA, et al. 1995. J. Exp. Med. 181:2213.
2. Robinson WH, et al. 1995. J. Immunol. 155:4739.
3. Hassan NJ, et al. 2004. Eur. J. Immunol. 34:930.
4. Gimferrer I, et al. 2004. J. Immunol. 173:2262.
5. Sarrias MR, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:11724.
6. Kofler DM, et al. 2011. J. Immunol. 187:3286.
7. Enyindah-Asonye G, et al. 2017. Proc. Natl. Acad. Sci. USA. 114:E6912.

Gene ID
923 View all products for this Gene ID
UniProt
View information about CD6 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 0    Revision Date: 10/10/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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