Purified Mouse IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG2a-53 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MG2a-53_GoChIP_IgG2a_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2a-53_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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401501 50 µg 72€
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401502 500 µg 222€
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Description

The isotype of MG2a-53 immunoglobulin is mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
ChIP - Verified
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401507-401512).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149.
  2. Lee H, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:2885. PubMed
  3. Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
Product Citations
  1. Lee H, et al. 2014. Invest Ophthalmol Vis Sci . 55:2885. PubMed
  2. Baker G, et al. 2014. Cancer Res. 74:5079. PubMed
  3. Wang J, et al. 2020. Cell. 183:1264. PubMed
  4. Ghosh R, et al. 2018. PLoS One. 13:e0194611. PubMed
  5. Gu C, et al. 2019. J Immunol. 203:389. PubMed
  6. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  7. Liu PY, et al. 2019. Nat Commun. 3.906944444. PubMed
  8. Zhou J, et al. 2020. Nat Commun. 0.625. PubMed
  9. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  10. Frasca D, et al. 2020. Front Immunol. 11:392. PubMed
RRID
AB_2800437 (BioLegend Cat. No. 401502)

Antigen Details

Gene ID
NA

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

Other Formats

View All Reagents
Description Clone Applications
Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Ctrl MG2a-53 ChIP, FC
Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl MG2a-53 FC, ICFC, WB, IP, ICC, IHC, FA
Purified Mouse IgG2a, κ Isotype Ctrl MG2a-53 FC, ICFC, WB, IP, ICC, IHC, FA, ChIP
Go To Top Version: 2    Revision Date: 08/24/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl

  • Purified Mouse IgG2a, κ Isotype Ctrl

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