Purified anti-SWAP70 Antibody

Pricing & Availability
Clone
9D9A19 (See other available formats)
Other Names
SWAP switching B-cell complex 70 kD subunit, switch-associated protein 70
Isotype
Mouse IgG1, κ
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Product Citations
publications
9D9A19_PURE_SWAP70_Antibody_1_WB_060816
Raji cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-SWAP70 (clone 9D9A19) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 9D9A19_PURE_SWAP70_Antibody_1_WB_060816
    Raji cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-SWAP70 (clone 9D9A19) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 9D9A19_PURE_SWAP70_Antibody_2_IP_060816
    Immunoprecipitation of SWAP70 from Raji cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-SWAP70 antibody (clone 9D9A19, lane 4). Western blot was performed using anti-SWAP70 antibody (clone 9D9A19). The astrostar indicates immunoglobulin heavy chain and light chain.
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654702 100 µg 360€
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Description

SWAP70 was initially isolated as a component of an isotype switch recombination complex (SWAP) from activated B lymphocytes. It translocates from cytoplasm to the nucleus upon B cell activation. SWAP70 interacts with F-actin and RAC, binds to PI(3,4)P2, and regulates membrane ruffling and lamellipodia formation in response to growth factor stimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human SWAP70 recombinant protein (89-585 aa).
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
Affinity purified.
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10-30 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2561890 (BioLegend Cat. No. 654702)

Antigen Details

Structure
Contains a PH domain, which is required for binding of phosphatidylinositol 3,4,5-trisphosphate, a coil-coil domain, and a domain weakly homologous to DH domains. Theoretical molecular weight is 69 kD.
Distribution
SWAP70 is localized to cytoplasm in resting B-cells, recruited to plasma membrane, and translocated to the nucleus upon B-cell activation.
Function
SWAP70 is a guanine nucleotide exchange factor (GEF) which binds phosphatidylinositol trisphosphate and transduces signals from tyrosine kinase receptors to RAC. SWAP70 is required for membrane ruffling and lamellipodia formation during cytoskeletal F-actin rearrangement.
Interaction
Interacts with cytoskeletal F-actin and Rac to regulate actin remodeling. Associates with NPM1, NCL, and PARP1 in switch-associated protein complex.
Cell Type
B cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction
Antigen References

1. Masat L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:2180.
2. Hilpelä P, et al. 2003. Mol. Biol. Cell. 14:3242.
3. Ihara S, et al. 2006. J. Cell Sci. 119:500.
4. Shinohara M, et al. 2002. Nature 416:759.
5. Pearce G, et al. 2006. Nat. Immunol. 7:827.

Gene ID
23075 View all products for this Gene ID
UniProt
View information about SWAP70 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/08/2016

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