Purified anti-STAT3 Phospho (Tyr705) Recombinant Antibody

Pricing & Availability
Clone
A16002B.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16002BdotRec_PURE_STAT3_Phospho_Tyr705_RECOM_Antibody_1_061621.png
Total cell lysates (15 µg total protein) from A-431 cells serum starved and untreated (-) or stimulated with 100 ng/mL recombinant human EGF (Cat. No. 585508) for 5 minutes (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) purified anti-STAT3 Phospho (Tyr705) recombinant antibody (clone A16002B.Rec), for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at 2.0 µg/mL. Purified anti-STAT3 antibody (clone 4G4B45) (Cat. No. 678002), was used as a pan STAT3 control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • A16002BdotRec_PURE_STAT3_Phospho_Tyr705_RECOM_Antibody_1_061621.png
    Total cell lysates (15 µg total protein) from A-431 cells serum starved and untreated (-) or stimulated with 100 ng/mL recombinant human EGF (Cat. No. 585508) for 5 minutes (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) purified anti-STAT3 Phospho (Tyr705) recombinant antibody (clone A16002B.Rec), for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at 2.0 µg/mL. Purified anti-STAT3 antibody (clone 4G4B45) (Cat. No. 678002), was used as a pan STAT3 control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • A16002BdotRec_PURE_STAT3_Phospho_Tyr705_RECOM_Antibody_2_061621.png
    Total cell lysates (15 µg total protein) from NIH/3T3 cells untreated (-) or stimulated with 10 ng/mL recombinant mouse IL-6 (Cat. No. 575708) for 20 minutes (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) purified anti-STAT3 Phospho (Tyr705) recombinant antibody (clone A16002B.Rec) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 2.0 µg/mL. Purified anti-STAT3 pan antibody (clone 4G4B45) (Cat. No. 678002), was used as a pan STAT3 control at 1.0 µg/mL (1:500 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • A16002BdotRec_PURE_STAT3_Phospho_Tyr705_RECOM_Antibody_3_061621.png
    NIH/3T3 cells were untreated (panel A) or stimulated with 10 ng/mL recombinant mouse IL-6 (Cat. No. 575708) for 20 minutes (panel B). Cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold methanol, and blocked with 5% FBS for 60 minutes. Fixed cells were then intracellularly stained with 5 µg/mL (1:100 dilution) purified anti-STAT3 Phospho (Tyr705) recombinant antibody (clone A16002B.Rec) for 2 hours at room temperature, after which proteins were visualized with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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690451 25 µg 192,00€
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690452 100 µg 448,00€
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Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to STAT3 phosphorylated at tyrosine 705
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone shows high pan-reactivity to STAT3 when used for WB. BioLegend clone 13A3-1 is more suitable for phospho-specific detection of STAT3.

This recombinant antibody clone is derived from clone A16002B.

RRID
AB_2894516 (BioLegend Cat. No. 690451)
AB_2894516 (BioLegend Cat. No. 690452)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous

Function
STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. The small GTPase Rac1 binds and regulates activity of STAT3.
Ligand/Receptor
When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Akira S, et al. 1994. Cell. 77:63.
  2. Zhang X, et al. 1995. Science. 267:1990.
  3. Sanchez-Margalet V, et al. 2001. Cell Immunol. 211:30.
  4. Simon A, et al. 2000. Science. 290:144.
  5. Hoey T, et al. 1999. Adv Immunol. 71:145.
Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/16/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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