Purified anti-RPS6 Antibody

Pricing & Availability
Clone
A16009C (See other available formats)
Regulatory Status
RUO
Other Names
40S ribosomal protein S6, Ribosomal Protein S6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16009C_PURE_RPS6_Antibody_1_093016
Total cell lysates (15 µg protein) from HeLa, Jurkat and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.25 µg/mL purified anti-RPS6 (clone A16009C) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • A16009C_PURE_RPS6_Antibody_1_093016
    Total cell lysates (15 µg protein) from HeLa, Jurkat and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.25 µg/mL purified anti-RPS6 (clone A16009C) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • A16009C_Pure_RPS6_Antibody_2_050218
    Total lysates (15 µg protein) from 293T and 293T/RPS6 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-RPS6 antibody, clone A16009C (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • A16009C_Pure_RPS6_Antibody_3_040618
    293T (human embryonic kidney cell line) fixed with Fixation Buffer (Cat No. 420801), permeabilized with 10X Permeabilization Buffer (Cat No. 421002), and intracellularly stained with purified anti-RPS6 (clone A16009C) (filled histogram) or purified Mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • A16009C_PURE_RPS6_Antibody_4_080221.png
    IHC staining of purified anti-RPS6 antibody (clone A16009C) on formalin-fixed paraffin-embedded human thymus tissue. Following antigen retrieval using 10 mM Tris-base, 1 mM EDTA in deionized water, pH 9.0, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 50 µm
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691802 100 µg 344,00€
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Description

The eukaryotic ribosome is composed of 40S and 60S subunits, which is involved in the physiological process of protein translation. In many organisms, ribosomal proteins undergo various post-translational modifications, including phosphorylation, acetylation, methylation, O-linked b-N-acetylglucosaminylation, and ubiquitylation. Ribosomal proteinS6 (RPS6), the first identified ribosomal protein which can be phosphorylated, is a component of eukaryotic ribosomal 40S subunit. Functional studies of RPS6 phosphorylation suggested its role in the regulation of protein synthesis, cell proliferation, and glucose homeostasis. The phosphorylation of RPS6 can be triggered by multiple stimuli including growth factors, tumor promoting agents, and mitogens. The inhibition of RPS6 phosphorylation was found mainly under stress conditions such as hypoxia, heat shock, and hyperosmolarity. RPS6 phosphorylation is also commonly used as a marker for neuronal activity and a read out of mammalian target of rapamycin complex 1 (mTORC1) activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human S6 peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, KO/KD-WB, IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested.It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_2629833 (BioLegend Cat. No. 691802)

Antigen Details

Structure
249 amino acids with a predicted molecular weight of 28.7kD.
Distribution

Cytoplasm and nucleus.

Function
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
Interaction
NCBP1, PASK.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Kressler A, et al. 2010. Biochim. Biophys. Acta. 1803:673.
2. Biever A, et al. 2015. Front. Mol. Neurosci. 8:75.
3. Ventimiglia FA, Wool IG. 1974. Proc. Natl. Acad. Sci. USA 71:350.
4. Lastick SM, et al. 1977. Mol. Gen. Genet. 152:223.
5. Ruvinsky I, et al. 2005. Genes Dev. 19:2199.
6. Meyuhas O. 2008. Int. Rev. Cell. Mol. Biol. 268:1.
7. Meyuhas O. 2015. Int. Rev. Cell. Mol. Biol. 320:41.

Gene ID
NA
UniProt
View information about RPS6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08/02/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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