Purified anti-rabbit IgG Antibody

Pricing & Availability
Clone
6B9G9 (See other available formats)
Other Names
N0034
Isotype
Mouse IgG1, κ
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6B9G9_Purified_IgG_Antibody_1_111416
Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with purified anti-GAPDH (clone Poly6314, rabbit IgG) antibody (lane 1) and purified anti-β-actin (clone 2F1-1, mouse IgG) antibody (lane 2). After a 30 minute wash, both lanes were further probed with purified anti-rabbit IgG (clone 6B9G9) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • 6B9G9_Purified_IgG_Antibody_1_111416
    Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with purified anti-GAPDH (clone Poly6314, rabbit IgG) antibody (lane 1) and purified anti-β-actin (clone 2F1-1, mouse IgG) antibody (lane 2). After a 30 minute wash, both lanes were further probed with purified anti-rabbit IgG (clone 6B9G9) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • 6B9G9_Purified_IgG_Antibody_2_111416
    Immunofluorescence of HeLa cells without (A) or with (B-D) pericentrin Rabbit primary antibody (Cat. No. 923701). Rabbit IgG mouse antibody was used as the secondary antibody (Cat. No. 410404). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as tertiary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A-D) is 1/15. Concentrations for (A, B) is 1 µg/ml, (C) is 0.5 µg/ml, (D) is 0.2 µg/ml.
Cat # Size Price Quantity Avail. Save
410404 100 µg 440€
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Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Immunogen
Rabbit IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml (Dilution 1:500-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632606 (BioLegend Cat. No. 410404)

Antigen Details

Structure
IgG antibodies are large molecules of about 150 kD made of four peptide chains. It contains two identical class γ heavy chains of about 50 kD and two identical light chains of about 25 kD.
Distribution

Secreted, B cell surface.

Function
Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
Gene ID
NA
UniProt
View information about IgG on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/13/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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