Purified anti-Neurofilament H (NF-H) Antibody

Pricing & Availability
Clone
SMI 37 (See other available formats)
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
SMI-37_Purified_Neurofilament-H_Antibody_1_022719
IHC staining of purified anti-Neurofilament H (NF-H) antibody (clone SMI 37) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-37_Purified_Neurofilament-H_Antibody_1_022719
    IHC staining of purified anti-Neurofilament H (NF-H) antibody (clone SMI 37) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-37_Purified_Neurofilament-H_Antibody_2_022719
    IHC staining of purified anti-Neurofilament H (NF-H) antibody (clone SMI 37) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-37_Purified_Neurofilament-H_Antibody_3_022719
    IHC staining of purified anti-Neurofilament H (NF-H) antibody (clone SMI 37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-37_Purified_Neurofilament-H_Antibody_4_022719
    Western blot of purified anti-Neurofilament H (NF-H) antibody (clone SMI 37). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 10 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
835803 25 µg 152€
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835804 100 µg 376€
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Description

Neurofilaments (NFs) are ~10 nanometer, type IV intermediate filaments expressed in neurons. NFs are the major components of the neuronal cytoskeleton, and primarily function to provide structural support for axons and regulate axonal diameter. There are three major mammalian neurofilament subunits which are named based on their apparent molecular weight: 1) neurofilament light (NF-L, ~70 kD), 2) neurofilament medium (NF-M, ~145-160 kD), and 3) neurofilament heavy (NF-H, ~200-220 kD). Neurofilaments are extensively phosphorylated, and their phosphorylation status plays an important role in modulation of their function. Abnormal NF modifications, mutations, and accumulation have been associated with neurodegenerative diseases. NF immunostaining is commonly used as a diagnostic marker for neuropathology detection. NFs are also useful for differentiating neurons (positive for NF) from glia (negative for NF).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SMI 37 reacts with a non-phosphorylated epitope in neurofilament H (NF-H) of most mammalian species. Immunocytochemically, SMI 37 visualizes some neuronal cell bodies, dendrites and thick axons in the central and peripheral nervous systems. SMI 37 staining is more restricted than that seen with SMI 32 or SMI 38 and Neuronal cell bodies are intensely stained while neuronal processes staining is less prominent. This IgM antibody reacts with goat anti-mouse IgG via the light chains common to IgG and IgM.

Application References

(PubMed link indicates BioLegend citation)
  1. Petzold A, et al. 2011. Brain 134:464. (WB) PubMed
RRID
AB_2715862 (BioLegend Cat. No. 835803)
AB_2632627 (BioLegend Cat. No. 835804)

Antigen Details

Structure
Neurofilament H has an apparent molecular mass of 200-220 kD.
Distribution

Tissue distribution: CNS, peripheral nerves and glandular cells of prostate.
Cellular distribution: cytoskeleton, nucleus, cytosol, and mitochondrion.

Function
Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
4744 View all products for this Gene ID
UniProt
View information about Neurofilament H (NF-H) on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 10/31/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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