Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
AN-18 (See other available formats)
Other Names
IFN-gamma
Isotype
Rat IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
517901 50 µg 120€
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517902 500 µg 336€
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IFN-γ protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ELISPOT, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.25 - 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 3.9 to 500 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detecting antibody, and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.

 

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Prat M, et al. 1984. P. Natl. Acad. Sci. USA 81:4515. (IP)
  2. Kort JJ, et al. 2006. J. Neuroimmunol. 173:23. (ELISPOT) PubMed
  3. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  2. Shimba A 2018. Immunity. 48:286. PubMed
Publication Library
RRID
AB_10900246 (BioLegend Cat. No. 517901)
AB_10900809 (BioLegend Cat. No. 517902)

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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