Purified anti-mouse I-Ak (Aβk) Antibody

Pricing & Availability
Clone
10-3.6 (See other available formats)
Other Names
MHC class II
Isotype
Mouse (CWB) IgG2a, κ
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Product Citations
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10-3dot6_Pure_071108.jpg
C3H mouse splenocytes stained with 10-3.6 purified, followed by anti-mouse IgG2a FITC
  • 10-3dot6_Pure_071108.jpg
    C3H mouse splenocytes stained with 10-3.6 purified, followed by anti-mouse IgG2a FITC
Cat # Size Price Quantity Avail. Save
109902 500 µg 344€
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Description

The 10-3.6 antibody reacts with the β chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens and I-Ag7 of NOD mice; it does not react with other haplotypes (e.g., b, d, p, q).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse k haplotype, Cross-reacts with H-2 f,r,s haplotypes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g. b, d, p, q).

Application References

(PubMed link indicates BioLegend citation)
  1. Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
  2. Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
  3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
  4. Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
  5. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
  6. Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
  7. Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
RRID
AB_313451 (BioLegend Cat. No. 109902)

Antigen Details

Structure
β chain of MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14960 View all products for this Gene ID
UniProt
View information about I-Ak on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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